ABSTRACT
Rice blast is one of the most serious diseases in the world. The use of resistant cultivars is the most preferred means to control this disease. Resistance often breaks down due to emergence of new races; hence identification of novel resistance donors is indispensable. In this study, a panel of 80 released varieties from National Rice Research Institute, Cuttack was genotyped with 36 molecular markers that were linked to 36 different blast resistance genes, to investigate the varietal genetic diversity and molecular marker-trait association with blast resistance. The polymorphism information content of 36 loci varied from 0.11 to 0.37 with an average of 0.34. The cluster analysis and population structure categorized the 80 National Rice Research Institute released varieties (NRVs) into three major genetic groups. The principal co-ordinate analysis displays the distribution of resistant and moderately resistant NRVs into different groups. Analysis of molecular variance result demonstrated maximum (97%) diversity within populations and minimum (3%) diversity between populations. Among tested markers, two markers (RM7364 and pi21_79-3) corresponding tothe blast resistance genes (Pi56(t) and pi21) were significantly associated and explained a phenotypic variance of 4.9 to 5.1% with the blast resistance. These associated genes could be introgressed through marker-assisted to develop durable blast resistant rice varieties. The selected resistant NRVs could be good donors for the blast resistance in rice crop improvement research.
ABSTRACT
Background: This study was designed to compare the study of serum bilirubin and various other Liver related enzymes in different types of Jaundice in patients attending the Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha. Methods: The study includes total 50 subjects, out of which 20 were controls and 30 were Jaundice cases. Thirty jaundice cases were divided into 10(Pre-Hepatic), 10(Hepatic) & 10(Post-Hepatic) jaundice patients according to inclusion-exclusion criteria. Results: The results of our study show that the levels of Total Bilirubin, Direct Bilirubin & Indirect bilirubin were significantly raised in the Jaundice patients than that of control. This study was found that the other liver enzyme i.e. SGOT, SGPT & ALP were found to be increased than the control. Conclusion: In conclusion, the high serum bilirubin level is a marker of Jaundice. Increased direct bilirubin and alkaline phosphatase (ALP) specifically points towards obstructive Jaundice, Increased indirect bilirubin level points towards pre-hepatic (Hemolytic) jaundice whereas increased bilirubin (Direct & Indirect) along with SGOT & SGPT levels points towards hepatic jaundice.